This paper reports on research carried out to determine the settled as well as other types of cultural markers including interface design elements and cultural dimensions that are appropriate to be used for cultural-centered website design and localization. For this, research discussed in this paper builds upon the existing body of research in website design and anthropologists’ cultural dimensions. The research was performed in two phases: a first study was carried out to re-evaluate some pre-researched websites, and the second study was performed to evaluate and rank anthropologist’s cultural dimensions. The findings of both research studies were evaluated and compared against earlier research results in order to provide insight into the evolution of the use of cultural markers. The results, a grouping of the cultural markers into 5 levels can be used for designing cultural-centered websites.